Robert J. Hariri
Celularity Inc, USA
Title: Decellularized human placenta for tissue and tissue and organ regeneration
Biography
Biography: Robert J. Hariri
Abstract
Recellularization of organ derived decellularized native and functional vasculatures with stem cells or tissue specific cells have been regarded as a viable approach to engineer organ to overcome donor shortage in organ transplantations. Human placenta is a large viable organ with abundant vasculatures and variety of hematopoietic stem cells and non-hemapotietic stem cells. To take advantage of this easily obtainable resource, we explore the feasibility of using human placenta’s vasculature as a suitable source for tissue and organoid engineering. We developed a proprietary method of sequential detergent based perfusion process to decellularize an entire human placenta as well as single placenta cotyledons. We demonstrated that the decellularized placenta or single cotyledon maintain efficient circulation and are able to conduct fluid and distribute cells, indicating that the vasculature system remained intact and functional after decellularization. The decellularized human placental vasculature scaffold (DHPVS) were shown to support in vitro proliferation and function of cells derived from multiple tissues including human placental adherent cells (PDAC®), primary hepatocytes, thyroid tissue epithelial cells, insulin producing cells and endothelial cells for 3 weeks. PDAC® grown on DHPVS demonstrated enhanced adipogenic differentiation comparing with 2-D culture when exposed to adipogenic induction culture medium. Furthermore, PDAC® transduced with Luciferase grown on DHPVS were shown to maintain viability for 4 weeks after subcutaneously implanted in mice. These in vitro and in vivo data provide evidences that decellularized human placenta vasculature can support cell growth and differentiation and can be used as a scaffold platform for tissue and organ engineering