Todd K. Malan
Brigham Young University, USA
Title: Novel point of care, automated, and closed system for processing Stromal Vascular fraction either with or without Collagenase
Biography
Biography: Todd K. Malan
Abstract
Stromal Vascular Fraction (SVF) is a component of lipoaspirate that can serve as a rich source of multipotent elements with phenotypic and gene expression profiles similar to human Mesenchymal stem cells (hMSCs) and Pericytes. SVF enriched fat grafts have demonstrated improved survival as compared to native fat as well as significant wound and scar healing properties. Currently a reliable, automated, and entirely closed point of care system for SVF processing technique does not exist. Here, we present the Q-Graft device from Human Med AG. The Q-Graft is an entirely closed and sterile point of care automated SVF processing device that can be used either with or without collagenase digestion. Adipose tissue is harvested directly into the device which is placed within the sterile OR field. The device then automates the incubation, filtration, washing, and suspension of the SVF pellet. We will present pre-clinical data gathered in our testing of the Q-Graft device to identify the cellular characterization of SVF product, growth kinetics and self-renewal assay, differentiation potential, ability to maintain sterility, and measures of residual collagenase. Comparisons will also be made in using the device either with or without collagenase.An evaluation as to the feasibility of direct point of care use of the Q-Graft device as an alternative to currently available manual and automated processing techniques will also be discussed.